Active Air Sampling Definition

After sampling the filter is placed directly on a medium in a standard 90mm petri dish and incubated.

Active air sampling definition.

Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter. However current active air sampling technology can be more advantageous and effective in assessing airborne viable contamination in cleanrooms than settle plate monitoring. The airborne contaminants are measured and compared against the volume of air and the results are stated as a concentration usually as milligrams per cubic meter mg m 3 or parts per million ppm. Active sampling requires the use of a pumping device to actively pass air through an air sample container whereas passive sampling does not.

During the sampling procedure the air sampler draws a defined air volume through the gelatin filter flow rate selectable between 2 and 8m 3 h. Any micro organisms contained in the sampled air are retained on the gelatin filter. The collected culture can then be incubated and analyzed ie count bacterial and or fungal colony forming units cfu and identify if required. Many samplers are subject to effects of temperature sampling duration wind speed and air concentrations.

In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time. Also a long sampling time is often required in order to get enough mass for detection. Given that both methods are designed to assess viable airborne contamination in cleanrooms there may be no advantage in performing these two parallel methods for the. Passive air sampling passive air sampling i e settle plates is a frequently.

Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane. There are two main types of air sampling that can be conducted to estimate the level of workers exposure to a certain contaminant stationary or area monitoring and personal chemical exposure monitoring. Further it is a challenge to measure non volatile species by passive sampler due to the low diffusion of particle to the adsorption medium. This leaves determination of the number ofsites for passive air sampling and surface sampling.

Passive sampling relies on the kinetic energy of gas molecules and diffusion of the gases in an enclosed space onto a sorbent medium. Air particulate sampling or air monitoring is essential for quality control qc purposes especially in companies that manufacture pharmaceutical products in controlled areas or clean rooms with filtered air. Well what pharmacists health care practitioners and microbiologists refer to as air sampling can simply be defined as taking a correct measurement of the practical airborne bacteria mold yeast spores and fungal cells which can be collectively referred to as bioburden in the. Active air sampling sites should be done at the same locations or as close as practical to avoid compro mising the other measure or the product integrity.